Segmentation of lipid nanoparticles from cryogenic electron microscopy images
Lipid nanoparticles (LNPs) were prepared as described (https://doi.org/10.1038/s42003-021-02441-2) using the lipids DLin-KC2-DMA, DSPC, cholesterol, and PEG-DMG2000 at mol ratios of 50:10:38.5:1.5. Four sample types were prepared: LNPs in the presence and absence of RNA, and with LNPs ejected into pH 4 and pH 7.4 …